Abstract: Pectinase (polygalacturomase,Ec3.2.1.15 ) from Aspergillus oryzae ac-491 was im1Tlobilized by a wide range of supports .the most promising system provde to be immobilization I on polyvinate alcohol (PVA)hydrogel.The optimal condition for enzyme immobilization was studied.The optimal pH and temperature of the immobilized enzyme were 3.8 and 50℃.respectively.The immobilized enzyme was stable at the range of pH304~5.0,not stable at the temperature higher than 50℃,but the temperature endurance of immobilized enzyme was higher than that of the natural pectinase

Key words: Pectinase immobilization Polyvinyl alcohol hydrogel