Abstract

Abstract: The determination of Va in Jin Hua Bergamot was carried by TLC-Fluorescence.After abstracted- with ethyl ether and absorbed with silicqgel the ratio of cyclohexane: petroleum ether: ethyl ether(7.3:1) was used to spread out, then determined by 254nm UV monitor and extracted with C4H9OH, the excitation wavelength was 340nm and determine the 480nm fluorescent for VA.The precision and the accuracy was well.The standard curve regression equation was Y=0.9524+60.46X .The linerar ranger of density was 0.25～1.25 μ g and the variation coefficient (r) was 7.36 .The relation coefficient was 0.9951.

Key words: , Vitamin A； Bergamot； TLC-Fluorimetry.；

Jin-Xiao-Ling, He-Hang-Ying, HU Yun-Qin. [J]. FOOD SCIENCE, 1999, 20(3): 54-56.

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