Abstract: In this paper, we compared the hematoxylin- eosin staining method, typan blue staining and giemsa staininmsethods to detectin vitro the EJ cell apoptosis induced by tea polyphenols. DNA gel electrophoresis method was used to make sure of apoptosis result induced by the same concentration of tea polyphenols. Typical cell apoptosis structure “ DNA ladder" was found in the EJ cell culture apoptosis induced by the tea polyphenols￡¨ 500|ì g/ml￡(c) . Typical cell apoptosis structure was all found in the three methods apoptosis cell staining . But the hematoxylin- eosin staining of the apoptosis cell showed more clearly than the other two. The conclusion was that DNA gel electrophoresis method with hematoxylin- eosin staining to detect EJ cell apoptosis induced by tea polyphenols was the best method of the three.

Key words: Polyphenols Hematoxylin- eosin staining Bladder cancer Apoptosis