FOOD SCIENCE ›› 2001, Vol. 22 ›› Issue (12): 59-61.

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Ascorbic Acid Determined with Fe(Ⅲ) -PAN-S System by Spectrophotometic Method

   Wu-Xiao-Hua, MIAO  Ji-Gen,   Wu-Lan-Ju   

  • Online:2001-12-15 Published:2012-02-14

Abstract: As ascorbic acid can rdeuce Fe(Ⅲ)-PAN-S to Fe(Ⅱ)-PAN-S.We used spectriphotmetic method to detiermine ascorbic acid of the fruits in this paper.Fe(Ⅱ)-PAN-S comple has main high peaks at 549nm and 748nm in HAc-NaAc buffer solution at pH=4.5.At 748nm,the content of ascorbic acid (in 0-100μg/25ml)is just in accordance with Beer’s law.It is very convenience and has high selection using this method,especially for the determination of ascorbic acid of the fruits.

Key words: Ascorbid acid Iron Spectrophotometic method