FOOD SCIENCE ›› 2001, Vol. 22 ›› Issue (8): 37-40.

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Enzymatic hydrolysis of casein to phospopeptides Ⅱ.Hydrolysate analysis and kinetic model

 QI  Wei,   He-Zhi-Min,   Shi-De-Qing   

  • Online:2001-08-15 Published:2012-02-13

Abstract:  Molecular weight distribution of casein-alcalase hydrolysates was analyzed by HPSEC.Under the optimal operating conditions,the kinetic constants were experimentally determined as Km=0.3132g/L,Kcat=12.9248/min,Ks=9.5565g/L,Kp=0.00285g/L.Taking into account the substrate inhibition,product inhibition and enzyme inactivation,a simplified kinetic model was established.The regressive results indicated that the average relative error was 6.21%.

Key words:  , Casein Alcalase Phospopeptides HPSEC Kinetic model;