Abstract: A Filamental Fungus has been seperated and screened in our laboratory.It could be used in liquid fermentation to transport elution material from Gardenia yellow to Gardenia blue.Orthogonal experiment showed that the optimum Gardenia blue culture medium and culture condition swere:peptone 4％, soybean extract 1％, elution material 7％, pH8.0; culture temperature 31℃, inoculum 5％(V/V),culture broth quantity 200ml/500ml flask, shake rotation 200 r/min and fermentation period 96 hr. The culture can produce 12g Gardenia blue pigment (E1％595≥50) per liter fermented broth.

Key words: Gardenia blue Liquid fermentation Orthogonal experiment