Studies on Rebuilding Cholesterol-Degrading Lactic Acid Bacteria Using the Protoplast Fusion Technique--The Fusion Hybrids Defined and the Ability of Cholesterol-Degrading Observed

Abstract

Abstract: Fusion hybrids C-T24-8 and S-T13-37 were rebuilf by using the protoplast fusion technique.Affter Comparing the ability of cholesterol-translating,the ability of skim milk solidifying,the size,the physiologic characters,the biochemical characters and the G+C%of DNA and so on,it defined eventually that C-T24-8,and S-T13-37 were fusion hybrids of C3 and T12-1,ST and T12-1,Their rates of cholesterol-degrading were 54-78%,23-45%in medium of PY-CHO and food respectively.

Key words: Protoplast fusion , Define , Utilization

WANG Cheng-Tao, NIU Tian-Gui. Studies on Rebuilding Cholesterol-Degrading Lactic Acid Bacteria Using the Protoplast Fusion Technique--The Fusion Hybrids Defined and the Ability of Cholesterol-Degrading Observed[J]. FOOD SCIENCE, 2002, 23(3): 27-30.

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Studies on Rebuilding Cholesterol-Degrading Lactic Acid Bacteria Using the Protoplast Fusion Technique--The Fusion Hybrids Defined and the Ability of Cholesterol-Degrading Observed

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