Abstract: The article was is to establish PCR method for assaying transgenic component 35S promoter derived from Cauliflower Mosaic Virus and NOS terminator derived from Agrobacterium tumefaciens in foods.According to the specific sequence of 35S and NOS Which have been used in transgenic crops frequently,two pairs of primers and two pairs of corresponding Fluorophore double-chain probes (FDCP) were designed to amplify parts of 35S promoter and NOS terminator,to allow for general screening of foods.PCR & Fluorescence PCR(FPCR)methods were established for the assay of transgenic component 35S and NOS.11 samples were tested with PCR & FPCR.The results showed that 5 samples were positive,6 samples were negative.The described methods enabled a sensitive、specific、simple and accurate assay of transgenic component and thus provided & a useful tool for routine analysis of raw and processed food products.

Key words: Transgenic , PCR , Fluorescence PCR , Fluorophore double-chain probe