Study on Purification and Structura l Characteristics of Polysaccharid e LBP2a from Fructus Lycii

Abstract

Abstract: LBP2a,one component ofLyciumbarbarumpolysaccharides(LBP),was obtainedfromFructuslycii by a series of steps of extraction,separation,and purification.LBP2a was o f homogeneous molecular weight assa yed by SDS -gel electrophoresis and longer Sephadex G -200column chromatograp hy.Its molecular weight was 77482as-sayed by SDS -gel electrophoresis.I t contained neutral sugar(69.3%),galacturnic acid(23.8%),and protein(5.3%).Monosaccharide residues identifi ed by GC -MS were rhamnose:xylose:arabinose:mannose:glucose:galactose(2.62:42.85:2.13:1.00:4.36:22.80).There wereβ-glycoside linkages as shown by FT -I Rspec-trum.Linkages betweensugars andam inoacids wereglycan -O -Seraccordi ngtobothits UVabsorptionchangeat240nmafterβ-eliminationreactions andits amin o acidcontentchange after the treatmentof0.2mol /LNaOH +1mol /L NaBH 4 .

Key words: Fructus lycii , Lyciumbarbarum polysaccharide(LBP), Purification , Structure

WANG Jian-Hua, WANG Jian-Min, LI Lin, ZHANG Min, ZHANG Sheng-Hua. Study on Purification and Structura l Characteristics of Polysaccharid e LBP2a from Fructus Lycii[J]. FOOD SCIENCE, 2002, 23(6): 44-48.

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Study on Purification and Structura l Characteristics of Polysaccharid e LBP2a from Fructus Lycii

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