Isolation and Identification of Glycopeptides in Soy Protein Hydrolysate

Abstract

Abstract: The purpose of the study was to isolate the peptide containing carbohydrates from soy protein hydrolysate, andto identify the peptide -carbohydrate linkage. β- Conglycincin was prepared from soy protein. The relative purity and thecarbohydrate content of it was 75.5% and 3.23%, respectively. And thenβ- conglycincin was hydrolyzed with Alcalase. Elutionpattern of β- conglycincin hydrolysate was obtained after it was analyzed through gel chromatography (Sephadex G-25).According to the elution patterns the hydrolysate was divided into two fractions P1 and P2. Results showed that the fraction P1was peptides containing carbohydrate about 8.23%. Thin-chromatography indicated that the peptides (P1) were combined withthe carbohydrates. Namely, fraction P1 was glycopeptides.

Key words: conglycincin, hydrolysis, isolation and identification, glycopeptides

ZHANG Xue-Mei, GUO Shun-Tang. Isolation and Identification of Glycopeptides in Soy Protein Hydrolysate[J]. FOOD SCIENCE, 2003, 24(10): 26-29.

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Isolation and Identification of Glycopeptides in Soy Protein Hydrolysate

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