Abstract: Optimal conditions for the extraction of flavonoids from propolis with water bath and ultrasonic wave were studiedby using orthoganol design. The results showed that the optimal conditions for the extraction with water bath were 75℃,25:1of 70% alcohol solution to propolis weight ratio and extraction for 1h. The optimal conditions with ultrasonic technique were20:1 of 75% alcohol solution to propolis weight ratio and extraction for 20 minutes. So the ultrasonic technique used gave betterresults than water bath in this work. The content of flavonoids in the extraction liquid was as high as 45.5%.

Key words: propolis, flavonoids, orthoganol design, ultrasonic wave, water bath, extracting