FOOD SCIENCE ›› 2003, Vol. 24 ›› Issue (5): 84-88.

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Study on Extraction, Purification and Antimicrobial Activity Investigation of Luteolin and Other Components in Peanut Hulls

 CHEN  Chun-Tao, MA  Qing-Yi, GAO  Yu-Mei, SUN  Hong-Yan   

  • Online:2003-05-15 Published:2011-12-13

Abstract: In this experiment the flavonoids in peanut hulls with strong antimicrobial activity were found in ethyl acetatecomponent by methanol extraction and then successive polarity-increase solvent extraction to monitor the antimicrobial effect.Through base-acid precipitation, and TLC purification three compounds (1# 2# 3#) were obtained and 2# was confirmed by UVchemical structure analysis as Luteolin (the yield was 0.53 %) to have antimicrobial activities against Staphylococcus Aurous andBacillus subtilis , while 3# to have activities against Staphylococcus Aurous and Saccharomyces. 1# should even broader antimicrobialeffects on Staphylococcus Aurous,Saccharomyces , Bacillus subtilis,and Penicilliam. According to UV, 1# might belong toflavanones, and need to be determined further.

Key words: Luteolin, TLC purification, antimicrobial activity