Abstract: The effects of various factors to produce of transglutaminase by L-18’ were studied. On the base of single factorexperiments, the optimum conditions for liquid fermentation were found to be: pH 7.5 , 30℃,the proper starter age 48 hours andproper inoculating amount 1%(v/v).The components of medium were: 0.8% glycerol, 0.4% tryptone, 0.6%yeast extract, 100×10-6 CaCl2, 500×10-6 K2HPO4 , and 0.1%Tween40. The transglutaminase activity reached 1.623U/ml.

Key words: Streptorerticillium griseovertici llatum L-18’, transglutaminase, fermentation conditions