Abstract: The ovum oil of Rana chensinensis was obtained by the methods of supercritical CO2 fluid and petroleum etherextraction, and was converted to methyl easter. The components of its fatty acid were determined by quartz capillary GC/MS.19 kinds of fatty acids were separated from the oil obtained by the methods of petroleum ether extraction and 72.38% of themare unsaturated fatty acids. 26 kinds of fatty acids were separated from the oil obtained by the methods of supercritical CO2 fluidextraction and 69.65% of them are unsaturated fatty acids. The study result indicates that the main components and content ofthe fatty acid in the oil obtained by the two kinds of methods are basically same, and the main unsaturated fatty acids arehexadecenoic acid, linoleic acid, oleic acid, eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA).

Key words: ovum oil of Rana chensinensis, fatty acid, component, GC/MS