Studies on the Fermentation Conditions of Pseudomonas fluorescens AR4 Producing 2-Keto-D-gluconic Acid

Abstract

Abstract: Using saccharide from corn starch hydrolysate as a sole carbon source, the factors (nitrogen sources, carbon-nitrogenratio, metal ions, the volume of inoculation, initial pH value of the medium, aeration rate and temperature) affecting 2-keto-D-gluconic acid fermentation by bacteriophage-resistant mutant Pseudomonas fluorescens AR4 were investigated. The resultsshowed that the production of 2-keto-D-glluconic acid was significantly affected by nitrogen sources, carbon-nitrogen ratio,initial pH value of the medium, aeration rate and temperature. Under the suitable fermentation conditions, the acid yield and theconversion rate was about 13.5% and 90% respectively.

Key words: Pseudomonas fluorescens AR4, 2-keto-D-gluconic acid, fermentation, bacteriophage-resistant mutant

SUN Wen-Jing, YANG Qing-Wen, HUANG Hui-Ying, GUO Jin-Quan, ZHAO Feng-Mei, LIU Jing-Ze. Studies on the Fermentation Conditions of Pseudomonas fluorescens AR4 Producing 2-Keto-D-gluconic Acid[J]. FOOD SCIENCE, 2004, 25(10): 46-49.

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Studies on the Fermentation Conditions of Pseudomonas fluorescens AR4 Producing 2-Keto-D-gluconic Acid

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