FOOD SCIENCE ›› 2004, Vol. 25 ›› Issue (11): 173-176.

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Optimization of Exopolysaccharide Production by Lactococcus FML02-8

 CHEN  Xiao-Hong, DONG  Ming-Sheng, ZHOU  Jian-Zhong, LIU  Xiao-Li   

  1. Lab of Food Microbe, College of Food Science and Technology, Nanjing Agricultural University
  • Online:2005-11-15 Published:2011-10-24

Abstract: The optimum condttion for the synthesis of EPS from FML02-8 was selected as follows: By means of 24 factorialdesign in SAS8.0 software, remarkable factors affecting the production of EPS were selected as temperature. pH and time.Through a central composite design, a preferable model of the production of EPS was established and tested. The constant pHin 3L fermentation indicated that different constant pH values during fermentation affect the yield of EPS from FML02-8. Underthe constant pH5.5, the yield of EPS was 503.3mg/L, increasing 30% compared without control of pH.

Key words: lactococcus, exopolysaccharide(EPS), response surface design, condition optimization