Study on Preparation and Purification of Cyclodextrin Glucantransfease of Alkalophilic Bacillus sp.7-12

Abstract

Abstract: Cyclodextrin glucantransferase (EC 2.4.1.19) of Alkalophilic Bacillus sp.7-12 was purified by ammonium sulfatefractionation, DEAE-cellulose column chromatography and sepharose CL-6B column chromatography. The final preparationthus obtained was purifed enzyme with molecular weight as 69,000D by means of SDS polyacrylamide gel electrophoresis. Theenzyme showed a Km of 1.24 mg/ml and Vmax 114.9μg/min when potato was used as substrate. The optimum conditions for activitywere temperature 60℃,pH 5.0, stable in the range 6.0～10.0 and below 70℃. The enzyme activity was strongly inhibited byAg+、Cu2+、Mg2+、Al3+、Co2+、Zn2+、Fe2+and slightly inhibited by Sn2+、Mn2+. The other metal ions showed noinfluence on the enzyme activity.

Key words: Alkalophilic Bacillus, cyclodextrin glucantransferase, purification, characterization

CAO Xin-Zhi, JIN Zheng-Yu. Study on Preparation and Purification of Cyclodextrin Glucantransfease of Alkalophilic Bacillus sp.7-12[J]. FOOD SCIENCE, 2004, 25(4): 43-46.

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Study on Preparation and Purification of Cyclodextrin Glucantransfease of Alkalophilic Bacillus sp.7-12

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