Abstract: Strain Lactarius deliciosus was obtained from tissue and spore of wild Lactarius deliciosus. The study on cultivatingconditions was conducted. The results showed that glucose and sucrose were the optimal carbon sources, peptone and KNO3 werethe optimal nitrogen sources. The optimal pH value was 7.5 to 8.0 and the optimial temperature 26℃ for mycelium growth ofLactarius deliciosus. When cultured with pine tree timber sawdust or wet pine root or wood sawdust at 25℃ constant temperature,95% constant humidity and illuminatied cultivating conditions, mycelium of Lactarius deliciosus grew well and mushroom alsocould be polarized.

Key words: Lactarius deliciosus, strain breeding, edible and medical fungus, artifical culture