FOOD SCIENCE ›› 2004, Vol. 25 ›› Issue (8): 91-94.

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Studies on Fermentative Medium Optimum Formulation of Bifidobacterium Infantis

 DU  Peng, LUO  Wei, HUO  Gui-Cheng   

  1. College of Food Science, Northeast Agriculture University
  • Online:2004-08-15 Published:2011-10-24

Abstract: According to the nutritional needs of B.infantis, the ingredient and dosage of medium were optimized by single factorexperimentation and quadratic design respectively. The optimum conditions of fermentative medium were as follows: 1.67%soya peptone, 0.83% casein peptone, 0.5% lactose, 0.5% yeast extract, 0.7% oligofructose and 15% carrot juice. Growth curveswith optimized fermentative medium were determined, with the change of pH and optics density. According to the growthcurve, the terminal time was 12h with B.infantis reeching 2.18×109cfu/ml.

Key words:  B. infantis, fermentative medium, optimization