Effects of Casein Enzymatic Hydrolysates on the Cytotoxicity of Natural Killer Cells

Abstract

Abstract: Using spleen cells of BALB/c mouse and SP20 cells as effector cells and target cells respectively, this research studiedthe effects of casein enzymatic hydrolysates with different DH, or different molecular weight range on NK cell cytotoxicity,which was determined by LDH method. The result showed that casein enzymatic hydrolysates had a concentration dependentbiphasic effect on NK cell cytotoxicity to SP20 cells. Compared with lower DH(9.74%)hydrolysates, hydrolysates with mediumDH (18.54%) or higher DH (24.02%) showed higher effect. Hydrolysates with different molecular weights had different effectson NK cell cytotoxicity.

Key words: casein enzymatic hydrolysates, degree of hydrolysis (DH), molecular cut-off, natural killer cell, cytotoxicity

MAO Xue-Ying, NAN Qing-Xian , LI Ying-Hui, SONG Jin-Ping, ZHONG Ping. Effects of Casein Enzymatic Hydrolysates on the Cytotoxicity of Natural Killer Cells[J]. FOOD SCIENCE, 2004, 25(9): 184-187.

[1]	ZOU Zhaojia, ZHENG Jie, HUANG Caihuan, LIU Fu, OU Shiyi. Preparation and Cytotoxicity of Acrolein-Alanine Adduct [J]. FOOD SCIENCE, 2021, 42(9): 1-6.
[2]	ZHUO Yiyun, Lü Jing, LIU Ying, WANG Zilong, LIU Man, LIANG Hui. Nattokinase Alleviates Alcoholic Liver Injury and Modulates Immune Function in Rats [J]. FOOD SCIENCE, 2019, 40(7): 156-162.
[3]	WANG Wenjuan, CAI Xiaofang, TANG Jie, ZHANG Xirong, FENG Di. Application of in Vitro Bioassays in Safety Evaluation of Food Contact Materials: A Review [J]. FOOD SCIENCE, 2019, 40(15): 277-284.
[4]	WANG Haimei, LI Yingying, HOU Juncai, JIANG Chenggang, LI Dongfei, LU Jiayin, ZHAO Yuehan, PANG Shiyue. Biological Activity and Stability of New Peptide Derivative KW-WK from Bovine Lactoferricin [J]. FOOD SCIENCE, 2018, 39(20): 57-62.
[5]	WU Hao, WANG Yiying, ZHEN Tianyuan, LUO Dan, ZHANG Xiao, YANG Shaolan, WANG Chengrong. Optimization of Enzymatic Reaction between Chitosan and Gallic Acid for Enhanced Antioxidative Activity and Reduced Cytotoxicity [J]. FOOD SCIENCE, 2017, 38(2): 227-232.
[6]	YANG Longping, YAN Wenjing, HUANG Mingming, QIAO Weiwei, ZHANG Jianhao. Antimicrobial Activity of Lysozyme-Functionalized Gold Nanoparticles [J]. FOOD SCIENCE, 2017, 38(14): 84-90.
[7]	WU Juan, LIU Yiming, LI Xia, WANG Jingfeng. Cytotoxicological Test of Photoactivated Curcumin [J]. FOOD SCIENCE, 2016, 37(3): 205-210.
[8]	XIONG Tao, LIU Yanyan, HUANG Tao, HUANG Qiaofen. Acid, Bile Tolerance and Adhesion Properties of Lactobacillus paracasei NCU622 [J]. FOOD SCIENCE, 2015, 36(5): 93-98.
[9]	ZHENG Dong-dong1, BI Wang-lai1, WANG Hong-xun2, LIU Zhi-guo1, DING Hong-bo1, LI Rui1,3,*. stx Genotypes and Virulence Characteristics of E. coli O157:H7 Strains Isolated from Meat Products Commercialized in Wuhan [J]. FOOD SCIENCE, 2014, 35(8): 94-98.
[10]	YE Qiong-xian, MIAO Ai-qing, CHENG Yue, CHEN Jian-ping, WANG Dong-mei. Extraction, Analysis and Bioactivity of Dark Tea Polymeric Polyphenols Prepared from Camellia sinensis var. Baiye Dancong [J]. FOOD SCIENCE, 2014, 35(5): 69-74.
[11]	. Research advances about nonoxidation ethanol metabolic products----Fatty acid ethyl esters [J]. FOOD SCIENCE, 2013, 34(3): 290-293.
[12]	ZHANG Yi-fan，LIU Gong-guan，LIU Qian，LIU Xue-bo*. Inhibitory Effect of Eriodictyol on Free Radical-induced Damage to Biological Macromolecules and Its Cytotoxicity on HepG2 Cells [J]. FOOD SCIENCE, 2013, 34(17): 126-130.
[13]	. Effects of Bioactive Polysaccharides on Immunocompetence of Human NK Cells [J]. FOOD SCIENCE, 2013, 34(1): 315-318.
[14]	. Development and Optimization of a Specialized Multi-enzyme Complex for the Production of Soybean Oligopeptides with High Degree of Hydrolysis [J]. FOOD SCIENCE, 2012, 33(23): 254-258.
[15]	DOU Shen，LIAO Yong-hong，YANG Chun-xia，XU Man. Effect of Microwave Pre-treatment on Degree of Hydrolysis of Defatted Soybean Meal Protein [J]. FOOD SCIENCE, 2012, 33(2): 119-123.

Effects of Casein Enzymatic Hydrolysates on the Cytotoxicity of Natural Killer Cells

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments