Abstract: The anti-pathogenic activities of Chrysanthemum nankingense Hand. Mazz were evaluated against 6 pathogenicbacteria and 4 pathogenic fungi. Results indicated that the 3 extracts (A, B and C) of Chrysanthemum nankingense Hand. Mazzshowed strong inhibitory effects on these pathogens in a concentration-dependent manner. The extract A (volatile fraction) was evidently active both in inhibiting the bacteria and the fungi with 0.3%～0.5% (g/ml) of MIC values. The extract B and C (non-volatile fraction) exhibited a strong inhibition to the test bacteria at MIC values of 0.3%～0.5%, and a poor inhibition to thesefungi with the MIC of 0.7%～1.0%. Some main active components of Chrysanthemum nankingense Hand. Mazz were isolatedand elucidated as volatile oil, total flavoniod, ailanthin, and lactone.

Key words: Chrysanthemum nankingense Hand. Mazz, extraction, anti-pathogenic activity, pathogens, the minimum inhibitory concentration (MIC)