Abstract: A 1.5kb α-amylase gene was prepared by PCR with Saccharomycopsis fibuligera genomic DNA as the template.The gene, together with phosphoglycerate kinase1 promoter and ethanol dehydrogenase1 terminator from S. cerevisiae was clonedinto a yeast expression vector (YEp352), generating a recombinant plasmid pLA8. The recombinant plasmid was introduced intoS. cerevisiae and the transformants were screened on the medium in which starch was the sole carbon source. The average α-amylase activities of the transformants in cellular extract and culture supernatants were 0.3U/ml and 1.1U/ml respectively. Noα-amylase activity was detected in those of the host strain.

Key words: industrial strain of S. cerevisiae, amylase, gene cloning and expression