FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (1): 116-120.

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Study on Extraction and Separation of Polysaccharide of Morchella esculenta Cultured by Fermentation

 WU  Qiu-Li, AN  Jia-Yan   

  1. 1.College of Chemical Engineering and Technology,Tianjin University;2.College of Biology and Food Technology,Dalian Institute of Light Industry
  • Online:2005-01-15 Published:2011-09-19

Abstract: For improving the polysaccharide yield, the factors influencing the extraction and the separation of polysaccharide of Morchella esculenta cultured by submerged fermentation were studied. The optimum conditions of alcohol-precipitating exopolysaccharide were: 3 times alcohol; freezing time 24 hours; temperature -15℃ and pH 7. The optimum extracting conditions of endo-polysaccharides from mycelia were given below: adding 50 times volume water to dry mycelia; extracting time 120 min; temperature 85℃, pH 7 and extracting two times. The study proved that there were five kinds of components in exo- polysaccharides of Morchella esculenta and two kinds in endo-polysaccharides by Sephadex-150 column chromatograph.

Key words: Morchella esculenta, exopolysaccharide, endo-polysaccharide, extraction