Extraction,Isolation and Purification of Water-soluble Polysaccharides from Pyracantha fortuneana

Abstract

Abstract: The extraction, isolation and purification of water-soluble Pyracantha fortuneana polysaccharides were studied. The results indicated that the optimal extraction technology of Pyracantha fortuneana polysaccharides were 90℃, 9h and 10:1(W/W) of water to its fruit. Extraction rate of the polysaccharides was 3.5%. Then through isolating it with fractional precipitation and DE-52 column chromatography, six glycoconjugates, PP-A2, PP-A3, PP-A4, PP-B1, PP-B2 and PP-B3, were obtained. Sephadex G-200 gel filtration chromatography showed that PP-A2, PP-A3, PP-B2 were pure.

Key words: Pyracantha fortuneana, polysaccharides, extraction, isolation and purification

ZHANG Hai-Sheng, DUAN Yu-Feng, ZHOU Fang, YANG Feng. Extraction,Isolation and Purification of Water-soluble Polysaccharides from Pyracantha fortuneana[J]. FOOD SCIENCE, 2005, 26(1): 157-161.

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Extraction,Isolation and Purification of Water-soluble Polysaccharides from Pyracantha fortuneana

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