Analysis of the argB Sequence from Corynebacterium Glutamicum and the Pilot Study on the Expression of the Gene in Escherichia coli

Abstract

Abstract: The argB sequence was obtained by designing and synthesizing the pair of primer, according to the report of GenBank about the sequence of Corynebacterium glutamicum, assayed by the temperature grads PCR instrument. The argB gene was cloned into plasmid pGEM-T-Easy. The recombinant strain harboring the pGEM-argB plasmid was verified by PCR reaction, plasmid extraction with alkaline method, and digestion with restriction endonuclease BamHI and EcoRI and DNA sequencing. An expressing plasmid pGEX-argB was constructed by the directional cloning of the targeted DNA into the plasmid pGEX-4T-1.

Key words: Corynebacterium glutamcium, argB gene, clone, expression

CHEN Xue-Lan, XU Zheng-Hong, TAO Wen-Yi. Analysis of the argB Sequence from Corynebacterium Glutamicum and the Pilot Study on the Expression of the Gene in Escherichia coli[J]. FOOD SCIENCE, 2005, 26(1): 31-35.

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Analysis of the argB Sequence from Corynebacterium Glutamicum and the Pilot Study on the Expression of the Gene in Escherichia coli

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