FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (1): 67-70.

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Study on Fermentation Conditions of Cellulase Produced by Neurosoora crassa

 FENG  Xin, WANG  Dan, XIN  Li-Xia, SONG  Wen-Hua   

  1. 1.Department of Environment Science and Safe Engineering,Tianjin Institute of Technology;2.Department of Technology,Shandong Agricultural University;3.Department of Environment Science and Engineering,Nankai University
  • Online:2005-01-15 Published:2011-09-19

Abstract: In this paper,fermentation conditions of cellulase produced by Neurospora crassa in the shaking bottle were studied. The results showed that the optimum conditions were as follows: cultivating solution initial pH was 5.5, cultivating temperature 28℃, rotation speed 150r/min and cultivating time 96 h. The most suitable organic nitrogen source was soybean powder and the most suitable inorganic nitrogen source was NH4HSO4. It helped produc cellulase under the conditions of 3% corncob powder and 0.5% bran acid hydrolysis solution(0.6 mol/L).In the optimum conditions, CMCase activity was 10.34IU/ml and FPase was 0.71IU/ml.

Key words: Neurospora crassa, cellulase, fermentation, condition