Optimization of Conditions for Production of Hepatoprotective Isoflavones in Maackia amurensis Suspension Cell Cultures

Abstract

Abstract: In order to realize high accumulation of hepatoprotective isoflavones in cultured cells of Maackia amurensis, medium components were optimized through single factor test and the orthogonal experiment. The results showed that NO3, NH4, 2,4- D and BA were four main factors that exerted significant effects on isoflavone accumulation in cultured cells and the optimum combination was 1500mg/L KNO3, 200mg/L (NH4)2SO4 and 0.1mg/L BA, respectively. In optimized medium, the average isoflavone accumulation reached 19.3mg/L, which was 9.3 times higher than in original medium.

Key words: Maackia amurensis, cell suspension culture, medium optimization, isoflavone -+

SHI Wei, LUO Jian-Ping, LUO Kai. Optimization of Conditions for Production of Hepatoprotective Isoflavones in Maackia amurensis Suspension Cell Cultures[J]. FOOD SCIENCE, 2005, 26(10): 113-115.

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Optimization of Conditions for Production of Hepatoprotective Isoflavones in Maackia amurensis Suspension Cell Cultures

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