Medium Optimization for Production of Nuclease P1 in Penicillium citrinum by Response Surface Methodology

doi:10.7506/spkx1002-6630-201117059

Abstract

Abstract: Fractional factorial design (FFD), central composite design (CCD) and response surface methodology (RSM) were used to optimize the culture medium for nuclease P1 production by Penicillium citrinum. The results of fractional factorial design showed that ZnSO4, yeast extract and composite phosphate had a significant effect on nuclease P1 production. The steepest ascent design (SAD) made three significant factors approach the maximum response area. Three significant factors were optimized by CCD and RSM. The optimized culture medium was composed of 4% glucose, 0.681% yeast extract, 0.4% corn syrup, 0.45% soybean meal, 0.042% ZnSO4, 0.06% MgSO4, 0.06% CaCl2, and 0.068% composite phosphate (KH2PO4: K2HPO4 = 1:1). The activity of nuclease P1 from optimal culture medium was 563 U/mL, which revealed a 64% increase when compared with nuclease P1 activity (202 U/mL) from original fermentation medium.

Key words: nuclease P1, Penicillium citrinum, medium optimization, response surface methodology

CLC Number:

Q93.335

YU Chen，ZHANG Ya-xiong，ZHAO Jie，ZHU Chang-xiong，YANG Hai-zhen，YAO Juan. Medium Optimization for Production of Nuclease P1 in Penicillium citrinum by Response Surface Methodology[J]. FOOD SCIENCE, 2011, 32(17): 283-286.

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Medium Optimization for Production of Nuclease P1 in Penicillium citrinum by Response Surface Methodology

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