Study on Breeding and Fermentation Conditions of High-yield SOD from Sacdharomyces cerevisiace

Abstract

Abstract: A SOD high-yield mutant was filtrated out through UV treatment to protolast of Sacdharomyces cerevisiace. The SOD activity was 720U/g fresh cell. The medium and fermentation condition was studied. It showed that the optimal medium was consisted of 2% glucose, 9%(V/V) malt wort, 1%peptone, 1%yeast extract, CuSO4 75mmol/ml ZnSO4 25mmol/ml. The optimal culture conditions were as follows:initial pH 5.5, medium cubage 75ml/500ml, 15%concentration inoculated and culture time 24h. In this condition,the biomass was 5.39g/100ml, SOD activity was 1024U/g fresh cell and SOD production was 5520U/ 100ml, yield 103% times of original strain.

Key words: S.cerevisiae BUV094, mutagenesis, breed, fermentation condition, SOD production

YANG Ming-Yan, GUO Ai-Lian, SHEN Jian, ZHANG Xiao-Qi, HUANG Ji-Hong. Study on Breeding and Fermentation Conditions of High-yield SOD from Sacdharomyces cerevisiace[J]. FOOD SCIENCE, 2005, 26(10): 147-150.

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Study on Breeding and Fermentation Conditions of High-yield SOD from Sacdharomyces cerevisiace

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