FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (10): 62-66.

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Influences of Culture Conditions on Rhamnolipid Produced by Pseudomonas aeruginosa

 MA  Ge-Li, PENG  Xin-Bang, GAO  Li-Feng, CHAI  Jie-Yu   

  1. Food and Biotechnology Department, Zhengzhou Institute of Light Industry
  • Online:2005-10-15 Published:2011-09-25

Abstract: The effects of fermentation conditions on rhamnolipid produced by Pseudomonas aeruginosa were investigated through the single factor and orthogonal experiments. The results showed that: The optimized compositions of the medium were (g/L): NaNO3 8, KH2PO4 0.8, K2HPO4 1.0, MgSO4 2.5, MnSO4 0.015, FeSO4 0.02, yeast extract1.5, and olive oil14%. The optimum conditions were: 60ml in 250ml total volume, seed volume 10%(V/V); the temperature of the shaking table 35℃, rotating speed 150r/min, the initial pH7.5, the culture time of seed 1d, and the fermentation time 4d. Under the optimum conditions, the maximum yield of rhamnose was 21.06g/L which it was increased by 31.7%.

Key words: rhamnolipids, rhamnose, fermentation, Optimize