FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (10): 87-90.

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Study on Separation of Theaflavins Monomer by High-Speed Countercurrent Chromatography

 YANG  Zi-Yin, TU  You-Ying, ZHAO  Qin, HE  Pu-Ming   

  1. Department of Tea Science, Zhejiang University
  • Online:2005-10-15 Published:2011-09-25

Abstract: Different solvent systems, and pretreatment of NaHCO3 to remove the other pigments and the optimized conditions for the equipment have been discussed in the separation of theaflavin monomer by high-speed countercurrent chromatography (HSCCC). The results have showed that the solvent system of ethyl acetate/hexane/methanol/water (3:1:1:6) could separate the theaflavins and NaHCO3 was helpful to purify the theaflavins. In regards to the equipment parameters the injection amount of tea pigments was 200mg, velocity of mobile phase was 1.2ml/min and the rotate speed was 880r/min.

Key words: Theaflavin monomer, separation, high-speed countercurrent chromatography