Study on Competitive Enzyme-linked Immunosorbent Assay for Ochratoxin A Monoclonal Antibody Determination

Abstract

Abstract: Ochratoxin A (OTA) artificial antigen was synthesized by active ester method. A monoclonal antibody was prepared after being immune to mice. A competitive enzyme-linked immunosorbent assay was developed with it. The linear range of the assay was between 200pg/ml and 6000pg/ml and the lowest detectable amount of OTA was 150 pg/ml. Cross reactivity was 35% with ochratoxin B, and less than 0.01% with citrinin, patulin, aflatoxin B1, deoxynivalenol and zearalenone. Recovery of OTA from wheat spiked with OTA was 83% to 116% and the coefficient of variation was 9.4%～19.8%. The analysis of 23 domestic samples showed OTA levels ranging from 0.6μg/kg to 2.56μg/kg.

Key words: ochratoxin A, artificial antigen, monoclonal antibody, ELISA

LIU Ren-Rong, YU Zhou, HE Qing-Hua, XU Yang. Study on Competitive Enzyme-linked Immunosorbent Assay for Ochratoxin A Monoclonal Antibody Determination[J]. FOOD SCIENCE, 2005, 26(11): 174-177.

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Study on Competitive Enzyme-linked Immunosorbent Assay for Ochratoxin A Monoclonal Antibody Determination

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