FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (11): 39-42.
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YU Han-Song, PENG Shuai, XIE Yuan-Hong, HU Yao-Hui
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Abstract: To isolate total RNA fromS accharomyces cerevisiae by the method of TRIZOL reagent kit, the RNA purified was rather not complete, since it showed band 5s rRNA only. After changing several steps of the method, the results showed that there appeared three clear bands—28s rRNA, 18s rRNA and 5s rRNA respectively with little RNA decomposed. The ratio of A260/A280 was 1.961, and A260/A230 2.070, so the purity was high.
Key words: TRIZOL, Sacharomyces cerevisiae, RNA isolate
YU Han-Song, PENG Shuai, XIE Yuan-Hong, HU Yao-Hui. Study on Improvement of RNA Isolating Reagent Kit—TRIZOL[J]. FOOD SCIENCE, 2005, 26(11): 39-42.
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