FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (12): 176-178.

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Determination of Content of Oleanolic Acid in Mung Bean, Red Bean, Lotus Seed and Jujube Respectively by HPLC

 WU  Bo, ZHANG  Han-Jun   

  1. Hubei Key Laboratory for Processing and Transformation of Agricultural Product, Wuhan Polytechnic University
  • Online:2005-12-15 Published:2011-10-01

Abstract: Oleanolic acid was important bioactive components in mung bean, red bean, lotus seed and jujube. The contents of these compounds in food were determined by reversed-phase high performance liquid chromatography. Nucleodur C18 column (250mm×4.6mmi.d., 5μm)was used with ethanol-water (80:20) as the mobile phase. The good linear relationships were obtained under the optimum conditions for each component. The recoveries of the standards added were 96.7%~101.5%, while the relatively standard deviations were within the range of 0.64%~2.89%.

Key words: high performance liquid chromatography, oleanolic acid, food, mung bean, red bean, lotus seed, jujube