Separation and Identification of Synthetic Antigen of Diethylstilbestrol by Using HPLC-MS

Abstract

Abstract: Using liquid chromatography spectrometry (ESI), phase B (methyl alcohol), phase A (water), 0.3mlmin of flow rate, a linear gradient from 60% to 100% of phase B and eluate for 25 minutes on a Lichrospher C-18 column, diethylstilbestrol andi t’sd erivatesc is (tran)- diethylstilbestrol- mno- ether-butyrate-ethyl( DES-MEBE),d iethylstilbestrol-di-ether-butyrate- ethyl (DES-DEBE) were separated and identified. The conjugates of DES-MCPE with proteins as complete antigens were prepared by the mixed anhydride method and the conjugation rate of them were analyzed by UV scanning. The results demonstrated that the isomerization was present in cis and tans of DES and it’s derivates, which was effected obviously by polar solvents. And through calculation the conjugation rate was 10.

Key words: HPLCMS, separation, diethylstilbestrol, synthetic antigen

ZHU Dong-Hui, XU Chuan-Lai. Separation and Identification of Synthetic Antigen of Diethylstilbestrol by Using HPLC-MS[J]. FOOD SCIENCE, 2005, 26(12): 199-201.

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Separation and Identification of Synthetic Antigen of Diethylstilbestrol by Using HPLC-MS

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