Abstract: Technology of extracting proanthocynidins from sea buckthorn seed were studied. The dried defatted sea buckthorn seed was used as raw material. After investigating the single effect factor on proanthocyanidins extraction, the optimum factors were obtained. The central composite design and corresponding response surface analysis were used to obtain the optimal parameters of processing. The optimum conditions of extraction were as follows: the rate of solution phase to solid phase 10: 1, the temperature 21℃, extraction time 90min, pH value 5.1, and the concentration of ethanol 65%. The extraction yield was 5.84% on the dry weight basis of defatted sea buckthorn seed powder with the product purity as 39.18%.

Key words: proanthocyanidins, sea buckthorn seed, extraction, response surface analysis