Abstract: A rapid column high performance liquid chromatography (HPLC) method for the determination of Clenbuterol Hydrochloride in meat was studied. The Clenbuterol Hydrochloride was extracted from the sample with water by ultrasonic extraction. Then the Clenbuterol Hydrochloride was purified and enriched by solid phase extraction. The Clenbuterol Hydrochloride was separated on a ZORBAX Stable Bound (4.6×50 mm, 1.8 μm) rapid chromatographic column with methanol and 0.01 mol/L KH2PO4 35:65 as mobile phase at a flow-rate of 1.0 ml/min, and monitored with the photodiode array detector at 243 nm. The recoveries of the Clenbuterol Hydrochloride were 84%～98%, and the relative standard deviations were 1.5%～ 2.5%. This method was being applied to the determination of Clenbuterol Hydrochloride in meat with good results.

Key words: high performance liquid chromatography, Clenbuterol Hydrochloride, solid phase extraction, meat