FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (7): 185-188.

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Determination of TPS by Improvement of Anthrone-sulfuric Acid Method

 WANG  Li-Ming, XIA  Wen-Shui   

  1. Food School, Southern Yangtze University
  • Online:2005-07-15 Published:2011-09-19

Abstract: The procedure of colorimetric analysis of TPS was investigated in this paper. The results showed that the accuracyof determination was largely improved by processing with the following procedure: 4ml of 0.05% anthrone in concentrated sulfuricacid was added to 1ml TPS solution and heated 5min at 100℃. The absorbance was measured at 675nm after cooling for 30min,while galactose was used as the standard. The recovery of the procedure was 94.58%±1.68% and RSD was 0.548%(n=6).

Key words: tea polysaccharide, anthrone-sulfuric method, improvement