Breeding of Bacillis Subtilis Strain Produced High Fibrinolysin Activity by Induced-mutation with γ-ray and Diethyl Sulfate

FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (9): 134-137.

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Breeding of Bacillis Subtilis Strain Produced High Fibrinolysin Activity by Induced-mutation with γ-ray and Diethyl Sulfate

LU Feng-Xia , LU Zhao-Xin, BIE Xiao-Mei, LIANG Si-Yu

College of Food Science and Technology, Nanjing Agricultural University

Online:2005-09-15 Published:2011-09-20

Abstract

Abstract: Bacillus Subtilis FM-S2, as parental strain, was mutagenized withγ-ray and DES. 200 mutants were selected by the isolation on the milk protein plates from 2341 survival.mutants. Seven of mutants over 2000UK.U/ml were obtained by measuring the fibrinolysin activity. It was found that γ-DES36 strain had the highest fibrinolysin activity, which reached 2719.89±242.51UK.U/ml, and that it was stable for 10 generations.

Key words: Bacillus Subtilis, fibrinolysin, radiation, DES, mutants

LU Feng-Xia , LU Zhao-Xin, BIE Xiao-Mei, LIANG Si-Yu. Breeding of Bacillis Subtilis Strain Produced High Fibrinolysin Activity by Induced-mutation with γ-ray and Diethyl Sulfate[J]. FOOD SCIENCE, 2005, 26(9): 134-137.

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Breeding of Bacillis Subtilis Strain Produced High Fibrinolysin Activity by Induced-mutation with γ-ray and Diethyl Sulfate

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