Abstract: Peroxidase(POD), Lactic dehydrogenase(LDH) and Glucose-6-phosphate dehydrogenase(G6PDH) in pasteurized milk and ultra high temperature milk were analyzed utilizing with isoenzyme electrophoresis in order to distinguish pasteurized milk and ultra high temperature milk. The result showed that Peroxidase and Lactic dehydrogenase in milk after dealing with more than 85 degrees sterilization were totally passivated. While the activation of Glucose-6-phosphate dehydrogenase in pasteur- ized milk is still high. But after ultra high temperature sterilization the activity is being absolutely lost. So this method can be used to differentiate between pasteurized milk and ultra high temperature milk.

Key words: pasteurized milk, ultra high temperature milk, isoenzyme electrophoresis