The Preparation of Monoclonal Antibody Against Heavy Metal Ion Cadmium

FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (9): 350-353.

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The Preparation of Monoclonal Antibody Against Heavy Metal Ion Cadmium

TANG Yong, XIANG Jun-Jian, LUO Hui-Wu, DENG Ning, WANG Hong

Center of Antibody Engineering, Department of Bioengineering, Jinan University

Online:2005-09-15 Published:2011-09-20

Abstract

Abstract: Heavy metal ion cadmium was chelated with Isothiocyanobenzyl-EDTA (IEDTA) and linked with vector protein KLH to gain the completed antigen (Cd-IEDTA-KLH). Several Balb/C mouse were immunized with Cd-IEDTA-KLH to make hybridoma cells. The strain 1F6 which can produce monoclonal antibody against Cd-IEDTA steadily was obtained. The ascites was obtained by immunized mouse with 1F6 cells. The titer of the monoclonal antibody in the ascites was about 1:51200.

Key words: heavy metal, cadmium, monoclonal antibody

TANG Yong, XIANG Jun-Jian, LUO Hui-Wu, DENG Ning, WANG Hong. The Preparation of Monoclonal Antibody Against Heavy Metal Ion Cadmium[J]. FOOD SCIENCE, 2005, 26(9): 350-353.

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