Quantitative Determination of Total Flavonoids in Sisal Flower by UV Spectrophotometry

Abstract

Abstract: Using rutin as the standard sample, the flavonoids substances in sisal flower were determined through methyl alcohol ultrasonic extraction and ultraviolet spectrophotometer. The determination result shows : under the chosen experiment conditions, there was a linear relationship between the concentration of the rutin solution and absorption spectrum in the range of 1.00～30μg/ ml with correlation coefficient of 0.9980. The average recovery of this method was 98. 9%, and the relative standard deviation was0. 07%. The sample dilutes directly for determination.This analysis method develop was simple, fast and accurate.

Key words: sisal flower, total flavonoids, UV spectrophotometry

LIU Yu-Fen, XIA Hai-Tao, YANG Shu-Ping. Quantitative Determination of Total Flavonoids in Sisal Flower by UV Spectrophotometry[J]. FOOD SCIENCE, 2005, 26(9): 418-419.

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Quantitative Determination of Total Flavonoids in Sisal Flower by UV Spectrophotometry

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