A Preliminary Investigation into the Separation and Identification of Astaxanthin and Related Compounds by C30-HPLC-PDA

Abstract

Abstract: Astaxanthin was an important functional factor wild applied in food and pharmaceutical products with various disease-protection and disease - treatment functions. The analysis of astaxanthin and related compounds was a difficult task due to their poor peak shapes on RP-HPLC. In this study, a series of effort was made to develop a separation method of astaxanthin- like compounds on C30-HPLC-PDA, including the separations of astaxanthin and its di-esters, geometrical isomers and degradation products. Data from those experiments suggested that by acidic acetonitrile-water (mobile phase A) and MTBE (mobile phase B), fractions mentioned as above were able to be separated successfully. This result is going to form the possibility of their quantization on C30-HPLC-PDA.

Key words: , astaxanthin； C30 column； HPLC；

PEI Ling-Peng, HUI Bo-Di, LIU Rui, LI Jing. A Preliminary Investigation into the Separation and Identification of Astaxanthin and Related Compounds by C30-HPLC-PDA[J]. FOOD SCIENCE, 2006, 27(10): 323-326.

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A Preliminary Investigation into the Separation and Identification of Astaxanthin and Related Compounds by C30-HPLC-PDA

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