Study on Extraction by Ultrasonic-NaOH, Purification and Physicalcochemical Characteristic Assay of Polysaccharide on Repseed Meal

FOOD SCIENCE ›› 2006, Vol. 27 ›› Issue (10): 345-349.

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Study on Extraction by Ultrasonic-NaOH, Purification and Physicalcochemical Characteristic Assay of Polysaccharide on Repseed Meal

CHEN Hao, FENG Rui, SHEN Min, XUE Ai-Fang, WU Mou-Cheng

1.College of Science, Huazhong Agricultural University, Wuhan 430070, China; 2.College of Food Science and Technology , Huazhong Agricultural University, Wuhan 430070, China

Online:2006-10-15 Published:2011-11-16

Abstract

Abstract: The polysaccharide on repseed meal was extracted with ultrasonic-NaOH in this paper, the condition was optimized, and the protein was removed by Sevage, RSPS1～4 were obtained by purify RSPS by DEAE-Sephadex A-25 and Sephadex G- 100. The physical and chemical characteristic of RSPS1～4 were analyzed and the monosaccharide content of RSPS-2 and RSPS-3 were analyzed by HPLC.

Key words: , extraction with ultrasonic-NaOH； polysaccharide on rapseed meal； isolation and purification； physicalcochemical characteristic assay； monosaccharide component；

CHEN Hao, FENG Rui, SHEN Min, XUE Ai-Fang, WU Mou-Cheng. Study on Extraction by Ultrasonic-NaOH, Purification and Physicalcochemical Characteristic Assay of Polysaccharide on Repseed Meal[J]. FOOD SCIENCE, 2006, 27(10): 345-349.

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Study on Extraction by Ultrasonic-NaOH, Purification and Physicalcochemical Characteristic Assay of Polysaccharide on Repseed Meal

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