Abstract: Study was conducted on the effect of protein composition distribution in rape pollen, alkali concentration, pH value, time and temperature on the extraction percent of glutelins in rape pollen. The protein composition distribution of defatted and wall-broken rape pollen is albumin 34.66%, globulin 1.930%, prolamine 2.810%, glutelins 45.96%, complex protein 11.04%, and the major protein in rape pollen were albumin and glutelins. Through orthogonal test,the optimum conditions of extraction were obtained on the basis of single factor test, as follow: ratio of solid-liquid1:20, NaOH concentration 0.30%, pH value 11, time 75min, temperature 70℃.

Key words:  , rape pollen； glutelins； extraction；