Study on the Characteristics of Phenylalanine Ammonia-lyase in Yam

Abstract

Abstract: The kinetic characteristics of phenylalanine ammonia-lyase (PAL) in yam was studied. The results suggested that the optimum substrate concentration of PAL was 1mmol/L. The optimal pH range of PAL in H3BO2-NaOH buffer was from 7.6 to 9.2 and the optimum pH were 8.0, 8.8. PAL couldn’t bear alkali and acid, especially the former. The optimal temperature range of PAL was 35℃ to 55℃ and the optimum temperature was 45℃, its activity was easy blunt above 70℃. It indicated that PAL had some tolerance to high temperature. L-tyrosine and L-cysteine had inhibiting effects on yam PAL and the inhibitory effects increased with the rise in the concentration of the inhibitors. The inhibition by L-tyrosine was more strong and appeared to be competitive. L-cystine was no effect on the activity of the PAL.

Key words: , yam； phenylalanine ammonia-lyase； charactrics； inhibitor；

JIANG Li, Yuan-Huai-Bo, Zhang-Shi-Jie, Jiang-Han-Hu. Study on the Characteristics of Phenylalanine Ammonia-lyase in Yam[J]. FOOD SCIENCE, 2006, 27(10): 36-40.

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Study on the Characteristics of Phenylalanine Ammonia-lyase in Yam

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