Extraction of Total Triterpenoids from the Rhizoma of Alisma orientalis (Sam.) Juzep by Microwave Pretreatment

Abstract

Abstract: The extraction of total triterpenoids(TT) from the rhizoma of Alisma orientalis (Sam.) Juzep by microwave pretreatment technique has been explored. Its parameters, such as particle size of the rhizoma, concentration and quantity of pretreatment solvent, radiation time, extract time after microwave pretreatment and concentration of solvent used after micro- wave pretreatment,were analyzed by single-factor tests. The optimal pretreatment condition was obtained by orthogonal test. The suitable extraction conditions indicate that the 40-mesh powder of the rhizoma is extracted two times by reflux with 85% ethanol for 30min after pretreated by 50% ethanol used for 1.9ml per gram rhizoma powder and radiated 180s in microwave. The yield of TT is 1.784%.

Key words: , microwave pretreatment； Alisma orientalis； total triterpenoids(TT)； orthogonal test；

YI Xing, XIAO Xiao-Nian, Huang-Dan-Fei, Deng-Ying-Na. Extraction of Total Triterpenoids from the Rhizoma of Alisma orientalis (Sam.) Juzep by Microwave Pretreatment[J]. FOOD SCIENCE, 2006, 27(10): 384-387.

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Extraction of Total Triterpenoids from the Rhizoma of Alisma orientalis (Sam.) Juzep by Microwave Pretreatment

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