Abstract: The fluorescence method utilizes the principle that coliform decomposes 4-mehtyumbelliferyl-β-D-galactoside,causing 4-mehtyumbellifery dissociated, which will emit blue fluorescence when being illuminated with 336nm UV light. In the experiment, the nutriments needed by coliform are concentrated and vacuum-dried to produce disposable testing cuvettes and using these testing cuvettes, specificity, sensitivity and stability tests as well as a comparative study against other inspecting methods of coliform in vegetables are performed. The results show that this method is fast, simple, accurate and stable and is applicable for fast detection of coliform in vegetables.

Key words: fluorescence method, coliform, vegetable