Purification and Characterization of β-glucosidase from Lactic Acid Bacterium

Abstract

Abstract: The β-glucosidase was purified to homogeneity by fractional ammonium sulphate precipitation, ion-exchange chromatography on DEAE-52 and SephadexG-100 gel-filtration chromatography. By SDS-PAGE, the molecular weight of the enzyme was estimated to be 60kDa. The enzyme exhibited optimum activity at 40℃ and pH6.0. Its activity maintained relatively stable under the condition at 45℃, pH4.5～7.0, and strongly inhibited by Hg2+, Ag+.

Key words: , lactic acid bacterium； &beta, -glucosidase； purification； enzymic hydrolysis；

MENG Xian-Wen, Li-Chang-Biao, Liu-Chang-Jiang, Gao-Rong-Hai, ZHANG Chun-Hong, Zhao-Xiu-Hong, Zheng-Yan. Purification and Characterization of β-glucosidase from Lactic Acid Bacterium[J]. FOOD SCIENCE, 2006, 27(11): 116-119.

[1]	LIU Xiaohui, XU Lijuan, LI Yongge, LIU Huishuang, YANG Xuejuan, SUN Baoshan, LIU Jiang, WEI Ran. Simultaneous Determination of Non-Volatile Flavor Substances in Acid-Hydrolyzed Corn Protein Powder [J]. FOOD SCIENCE, 2016, 37(8): 152-158.
[2]	GAO Yi, ZHOU Guangming, ZHANG Caihong, YU Lu, CHEN Junhua, QIN Hongying. Simultaneous Determination of Six Active Components in Viola yedoensis Makino by HPLC [J]. FOOD SCIENCE, 2016, 37(6): 101-105.
[3]	CHU Zhong, WANG Hairu, ZHANG Yanjun, YAO Jing. GC-MS Analysis of Volatiles in Vanilla Husk Extracted by Headspace Solid-Phase Microextraction [J]. FOOD SCIENCE, 2016, 37(6): 126-131.
[4]	SONG Hui, GENG Zhiming, REN Shuang, WANG Daoying, ZHANG Muhan, SUN Chong, XU Weimin. Simultaneous Determination of 13-HODE, 9,10-DHODE, 9,10-EPODE and 9,10,13-THOD in Cured Meat Products by HPLC-MS/MS [J]. FOOD SCIENCE, 2016, 37(18): 133-140.
[5]	XIE Liyan, LIN Ying*, WU Heng, XU Xiaohui, ZHU Xueqiong. Interaction Mechanism between Acetylated Distarch Adipate and Soymilk on Yuba Membrane [J]. FOOD SCIENCE, 2015, 36(5): 77-82.
[6]	XIE Yuejie1, HE Zhifei2, LI Hongjun1,*. Volatile Flavor Profile of Rabbit Meat Extracted by Three Frequently Used Techniques [J]. FOOD SCIENCE, 2015, 36(24): 147-151.
[7]	ZHANG Xiaoli, MAO Lixin, FAN Sanhong. Synchronous Fluorescence Spectroscopy Combined with Principal Component Analysis to Identify Orange Juice Quality [J]. FOOD SCIENCE, 2015, 36(18): 162-166.
[8]	XIE Jilin, ZHANG Wei*, CHEN Xiaoquan, ZHAO Yahua, ZHU Xi. Analysis of Changes in Aroma Constituents during Storage of Ripe Pu’er Tea [J]. FOOD SCIENCE, 2015, 36(10): 154-157.
[9]	FANG Bowen, QI Ruiting, ZHANG Ying* . Active Components and Antioxidant Activities of Different Parts of Citrus paradise Fruit [J]. FOOD SCIENCE, 2015, 36(10): 158-163.
[10]	YUAN Jiangfeng1, ZHAO Junfeng1, SUN Junjie1, HE Lingmei2, ZHANG Zhiqi3, LIU He1. Determination of Flavonoids and Triterpene Acids in Forsythia suspensa Leaves from Henan and Shanxi Provinces [J]. FOOD SCIENCE, 2015, 36(10): 164-167.
[11]	WU Run-feng, ZHAO Li*, YUAN Mei-lan, SU Wei, CHEN Li-li, WEN Hui-fang, WANG Xiao-cheng. Quality Change of Surimi of Four Major Chinese Carps before and after Rinsing [J]. FOOD SCIENCE, 2014, 35(9): 132-136.
[12]	HE Yang-yang, ZENG Wei, WANG Qing-long, WANG Da-yun, CHEN Gui-guang, LIANG Zhi-qun*. Optimization of Medium Composition and Fermentation Conditions by Response Surface Methodology for the Production of Poly-γ-Glutamic Acid by Bacillus subtilis [J]. FOOD SCIENCE, 2014, 35(9): 147-151.
[13]	ZHU Sai-feng, WU Jian-feng, ZHAO Xi-rong. Response Surface Methodology for Optimization of Culture Conditions for the Production of Tetramethylpyrazine by Bacillus subtilis S0507 [J]. FOOD SCIENCE, 2014, 35(9): 218-223.
[14]	LIU Tao, SUN Jian, GUO Chen, ZHAO Xiao-hong. Comparison of Early Damage of CHO Cells in the Presence of Different Flavonoid Monomers [J]. FOOD SCIENCE, 2014, 35(11): 223-228.
[15]	. Anti-Tumor Activity of Purple Sweet Potato Juice and Its Apoptosis-Inducing Mechanism in HepG2 Cells [J]. FOOD SCIENCE, 2013, 34(1): 263-267.

Purification and Characterization of β-glucosidase from Lactic Acid Bacterium

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments