Expression of the Glucoamylase Gene of Aspergillus niger in Pseudomonas fluorescens

Abstract

Abstract: Aspergillus niger glucoamylase gene cDNA was subcloned into broad host expression vector pBBR1MCS-2 with correct reading frame to obtain recombinant vector pBBR1MCS-2GA. Then it was transformed into Pseudomonas fluorescens AR4 which was a high yield strain of 2-keto-D-gluconic acid,and obtained recombinant bacteria ARW4. SDS-PAGE gel electrophoresis and Western blot analysis showed that the recombinant glucoamylase protein had expressed effectively in ARW4,and existed in the form of inclusion which was inactive.

Key words: Aspergillus niger, glucoamylase gene, Pseudomonas fluorescens, prokaryotic expression

WANG Hui, Hu-Yu-Hong, Sun-Wen-Jing, WANG Zi-Wei, Liu-Jing-Ze. Expression of the Glucoamylase Gene of Aspergillus niger in Pseudomonas fluorescens[J]. FOOD SCIENCE, 2006, 27(11): 199-202.

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Expression of the Glucoamylase Gene of Aspergillus niger in Pseudomonas fluorescens

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